By: Muhammad Raznisyafiq Razak; Ahmad Zaharin Aris; Fatimah Md Yusoff et al.

Artikel Disediakan Oleh: Farah Izana Abdullah

A research group led by Muhammad Razak from the Faculty of Forestry and Environment (2022) described assessment of RNA extraction protocols from cladocerans. Conventional ecotoxicological assessment is highly costly, time-consuming and dependent on testing animals as surrogates for exposure effects on humans. The National Institutes of Health in the U.S. suggested eco toxicological testing using animal models should construct the assessment methodology using the Replacement, Reduction, and Refinement strategy. This study showed the usefulness of a well known inert carrier, glycogen, in enhancing the quantity, quality and integrity of RNA. Phenol-chloroform method is not compatible with M. micrura or other zooplankton, insects and molluscs species.The lysis reagent used in this method will eventually denature 28S rRNA.The analysis involved 10 individuals. They argue that preservation methods are essential to maintain the integrity of RNA from samples, determining the success of downstream analysis. Further studies by using several preservation methods before the RNA extraction are recommended.

Web: https://doi.org/10.1371/journal.pone.0264989 A

Date of Input: 20/02/2023 | Updated: 29/03/2023 | s_humaira