Peptida GR15 Daripada Arthrospira Platensis Menunjukkan Mekanisme Antioksidan In-vitro Dan In-vivo | INSTITUT ANTARABANGSA AKUAKULTUR DAN SAINS AKUATIK

» ARTIKEL » Peptida GR15 daripada Arthrospira platensis menunjukkan mekanisme antioksidan in-vitro dan in-vivo

Peptida GR15 daripada Arthrospira platensis menunjukkan mekanisme antioksidan in-vitro dan in-vivo

By: Manikandan Velayutham, Ajay Guru, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ki Choon Choi, Preetham Elumalai, Ramasamy Harikrishnan, Aziz Arshad and Jesu Arockiaraj

Article Prepared By: Farah Izana Abdullah

S-adenosylmethionine or known as SAM, AdoMet and SAMe is an essential cellular enzyme found in prokaryotes and eukaryotes. Arthrospira platensis contains potential source of peptides with various biological properties such as antioxidants. The GR15 peptide identified from protein S-adenosylmethionine synthase (SAMe) of A. platensis was investigated for its antioxidant property using cell-free assays in vitro and in vivo models. In this study, the potency of Reactive Oxygen Species (ROS) reduction efficiency of GR15 peptides against the H₂O₂ oxidative stress-induced MDCK cells and zebrafish larvae model and the upregulation of the glutathione family genes was investigated. GR15 peptide possessed to have potential antioxidant activity where it was effectively reduce the oxidative stress generated by H₂O₂ and does not exhibit any toxicity in MDCK cell and zebrafish embryos. GR15 enhanced the antioxidant property and also up-regulated the antioxidant gene, thus reduced the ROS level in both in vitro and in vivo models. Therefore, the GR15 peptide has the potential antioxidant ability; hence further research can be directed towards the therapeutic product or drug development against disease caused by oxidative stress.

Web: https://doi.org/10.1016/j.jbiotec.2021.10.010